FIGURE

Fig. 1

ID

ZDB-FIG-140523-3

Publication

Govindan et al., 2014 - Hapln1a is required for connexin43-dependent growth and patterning in the regenerating fin skeleton

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Fig. 1

Validation of microarray results by in situ hybridization using whole mount in situ hybridization on 5 dpa regenerating fins.

(A) hapln1a expression is reduced in sof ^b123 compared with WT (B). alf ^dty86 expression is variable (C,D). (E,F) In situ hybridization on a WT 5 dpa cryosection reveals compartment specific expression of hapln1a, mainly in the blastema (b), in the mesenchyme (m), and skeletal precursor cells (*). The thick arrow identifies the basal layer of the epidermis, which underlies the epidermis (e). The thin arrow identifies lepidotrichia and the arrowhead identifies the actinotrichia. The amputation plane is indicated in panels A-D. Scale bar in E and F is 50 μm.

Expression Data

Gene:	hapln1a
Fish:	WT
Condition:	physical alteration: anatomical structure
Anatomical Terms:	blastema regenerating tissue mesenchyme
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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