FIGURE

Fig. 2

ID

ZDB-FIG-140512-2

Publication

Xu et al., 2014 - Gbeta1 controls collective cell migration by regulating the protrusive activity of leader cells in the posterior lateral line primordium

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Fig. 2

Gβ1, but not Gβ4, is required for migration of the pLLP. (A-E) Epifluorescence images of 48-hpf Tg(-8.0cldnb:lynEGFP) control embryos (A), embryos injected with MOs targeting gnb1a (B, C), gnb1a/b (D) or gnb4a/b (E). All images are lateral views with anterior to the left. White arrowheads, NMs. The embryo was subdivided into six equal sections (zones 1-6) from the ear to the tip of the tail. (F) Quantification of pLLP migration relative to the position of the posterior-most deposited NM (red arrows in A-E). The number of animals/injection is indicated.

Expression Data

Gene:	EGFP
Fish:	zf106Tg zf106Tg + MO2-gnb1a + MO4-tp53 zf106Tg + MO1-gnb4a + MO1-gnb4b + MO4-tp53 zf106Tg + MO2-gnb1a + MO2-gnb1b + MO4-tp53
Knockdown Reagents:	MO1-gnb4a MO1-gnb4b MO2-gnb1a MO2-gnb1b MO4-tp53
Anatomical Terms:	lateral line neuromast posterior lateral line primordium
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf106Tg + MO2-gnb1a + MO4-tp53 zf106Tg + MO1-gnb4a + MO1-gnb4b + MO4-tp53 zf106Tg + MO2-gnb1a + MO2-gnb1b + MO4-tp53
Knockdown Reagents:	MO1-gnb4a MO1-gnb4b MO2-gnb1a MO2-gnb1b MO4-tp53
Observed In:	neuromast posterior lateral line neuromast deposition posterior lateral line neuromast primordium migration whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 385(2), Xu, H., Ye, D., Behra, M., Burgess, S., Chen, S., and Lin, F., Gbeta1 controls collective cell migration by regulating the protrusive activity of leader cells in the posterior lateral line primordium, 316-27, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.