FIGURE

Fig. 4

ID

ZDB-FIG-140417-11

Publication

Wang et al., 2013 - Targeted inactivation and identification of targets of the Gli2a transcription factor in the zebrafish

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Fig. 4

The Gli1 and Gli2a activators differ in their ability to counter Gli2a-mediated repression.

(A,B,E,F) gli1^ts269/+; gli2a^ty119/+ (C,G) gli2a^i276/ty119 (D,H), and gli1^ts269/+; gli2a^i276/ty119 (D) embryos at 24hpf stained with anti Prox1a (green) and either mAb F59 (red: panels A–D) or 4D9 (red: panels E–H) to reveal slow-twitch muscle and MP fibres. All panels show the caudal somites; the white dotted line indicates the position of the end of the yolk sac extension. The somites shown in panels C and G are located at 3–5 somites away from the end of the yolk sac extension. (I–L) Expression of myod1 in (I) gli2a^i276/ty119, (J) gli1^ts269/+; gli2a^ty119/+, (K) gli1^ts269/+; gli2a^i276/ty119, and (L) gli2a^ty119/ty119 embryos at 18 ss.

Expression Data

Gene:	myod1
Antibodies:	Ab-F59 Ab1-eng
Fish:	gli2a^ty119/ty119 gli2a^ty119/+; gli2a^i276/+ gli1^ts269/+; gli2a^ty119/+ gli1^ts269/+; gli2a^ty119/+; gli2a^i276/+
Anatomical Terms:	muscle pioneer slow muscle cell somite
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gli1^ts269/+; gli2a^ty119/+ gli1^ts269/+; gli2a^ty119/+; gli2a^i276/+
Observed In:	muscle pioneer slow muscle cell somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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