ZFIN ID: ZDB-FIG-140417-11
Wang et al., 2013 - Targeted inactivation and identification of targets of the Gli2a transcription factor in the zebrafish. Biology Open   2(11):1203-1213 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 4

The Gli1 and Gli2a activators differ in their ability to counter Gli2a-mediated repression.

(A,B,E,F) gli1ts269/+; gli2aty119/+ (C,G) gli2ai276/ty119 (D,H), and gli1ts269/+; gli2ai276/ty119 (D) embryos at 24hpf stained with anti Prox1a (green) and either mAb F59 (red: panels A–D) or 4D9 (red: panels E–H) to reveal slow-twitch muscle and MP fibres. All panels show the caudal somites; the white dotted line indicates the position of the end of the yolk sac extension. The somites shown in panels C and G are located at 3–5 somites away from the end of the yolk sac extension. (I–L) Expression of myod1 in (I) gli2ai276/ty119, (J) gli1ts269/+; gli2aty119/+, (K) gli1ts269/+; gli2ai276/ty119, and (L) gli2aty119/ty119 embryos at 18 ss.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 gli1ts269/+; gli2aty119/+ standard conditions 14-19 somites somite ISH
gli1ts269/+; gli2aty119/+; gli2ai276/+ standard conditions 14-19 somites somite ISH
gli2aty119/+; gli2ai276/+ standard conditions 14-19 somites somite ISH
gli2aty119/ty119 standard conditions 14-19 somites somite ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-F59 IHC gli1ts269/+; gli2aty119/+ standard conditions Prim-5 slow muscle cell
IHC gli1ts269/+; gli2aty119/+; gli2ai276/+ standard conditions Prim-5 Not Detected slow muscle cell
IHC gli2aty119/+; gli2ai276/+ standard conditions Prim-5 slow muscle cell
Ab1-eng IHC gli1ts269/+; gli2aty119/+ standard conditions Prim-5 muscle pioneer
IHC gli1ts269/+; gli2aty119/+; gli2ai276/+ standard conditions Prim-5 Not Detected muscle pioneer
IHC gli2aty119/+; gli2ai276/+ standard conditions Prim-5 muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
gli1ts269/+; gli2aty119/+ standard conditions Prim-5 somite lacks parts or has fewer parts of type muscle pioneer, abnormal
Prim-5 somite lacks parts or has fewer parts of type slow muscle cell, abnormal
gli1ts269/+; gli2aty119/+; gli2ai276/+ standard conditions Prim-5 somite lacks all parts of type muscle pioneer, abnormal
Prim-5 somite lacks all parts of type slow muscle cell, abnormal
Acknowledgments:
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