ZFIN ID: ZDB-FIG-140417-10
Wang et al., 2013 - Targeted inactivation and identification of targets of the Gli2a transcription factor in the zebrafish. Biology Open   2(11):1203-1213 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 3

Gli1 and Gli2a act redundantly to pattern the zebrafish myotome.

(A,E) Wild-type (WT); (B,F) gli2ai276; (C,G) gli1ts269 and (D,H) gli1ts269; gli2ai276 24hpf embryos stained with anti Prox1a (green) and either mAb F59 (red: panels A–D) or 4D9 (red: panels E–H) to reveal slow-twitch muscle and MP fibres. (I–K) Transverse sections of embryos shown in panels E–G. (L–O) Dorsal views of the caudal regions of 18ss embryos hybridized with a probe for myod1 transcript. (L) WT; (M) gli2ai276; (N) gli1ts269 (O) gli1ts269;gli2ai276.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 WT standard conditions 14-19 somites somite ISH
gli1ts269/ts269 standard conditions 14-19 somites somite ISH
gli1ts269/ts269; gli2ai276/i276 standard conditions 14-19 somites somite ISH
gli2ai276/i276 standard conditions 14-19 somites somite ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-F59 IHC WT standard conditions Prim-5 slow muscle cell
IHC gli1ts269/ts269 standard conditions Prim-5 slow muscle cell
IHC gli1ts269/ts269; gli2ai276/i276 standard conditions Prim-5 slow muscle cell
IHC gli2ai276/i276 standard conditions Prim-5 slow muscle cell
Ab1-eng IHC WT standard conditions Prim-5 fast muscle cell
IHC Prim-5 muscle pioneer
IHC gli1ts269/ts269 standard conditions Prim-5 Not Detected fast muscle cell
IHC Prim-5 Not Detected muscle pioneer
IHC gli1ts269/ts269; gli2ai276/i276 standard conditions Prim-5 fast muscle cell
IHC Prim-5 muscle pioneer
IHC gli2ai276/i276 standard conditions Prim-5 fast muscle cell
IHC Prim-5 muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
gli1ts269/ts269; gli2ai276/i276 standard conditions Prim-5 somite has fewer parts of type slow muscle cell, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open