nrca14 cone photoreceptors have normal outer segments and connecting cilia.
(A–B) TEM images of 5 dpf WT (A) and nrca14 (B) cone photoreceptor OSs. There was no difference in OS appearance or length (G) between nrca14 and WT cone photoreceptors at 5 dpf (p = 0.7). (C–F) Confocal images of 5 dpf zebrafish larval retinas immunostained using antibodies against the CC proteins acetylated tubulin (C, D) or IFT88 (E, F). The number (I) and length (H) of acetylated tubulin stained CC was not significantly different between WT and nrca14 photoreceptors (p = 0.9 and 0.4 respectively). There was no significant difference (p = 0.7) in the number of IFT88 stained cilia between WT and nrca14 (J). Antibody staining is shown in yellow; nuclei were stained with Sytox Green (D, E) or Hoechst (G, H) and are shown in blue. Syn = photoreceptor synapses, IS = inner segment. Scale bar = 1 μm in A and B and 2 μm in C–F. Graphs show mean values, error bars are STDEV for multiple larvae.