ZFIN ID: ZDB-FIG-140403-16
George et al., 2014 - Synaptojanin 1 is required for endolysosomal trafficking of synaptic proteins in cone photoreceptor inner segments. PLoS One   9(1):e84394 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1

nrca14 cone photoreceptors have normal outer segments and connecting cilia.

(A–B) TEM images of 5 dpf WT (A) and nrca14 (B) cone photoreceptor OSs. There was no difference in OS appearance or length (G) between nrca14 and WT cone photoreceptors at 5 dpf (p = 0.7). (C–F) Confocal images of 5 dpf zebrafish larval retinas immunostained using antibodies against the CC proteins acetylated tubulin (C, D) or IFT88 (E, F). The number (I) and length (H) of acetylated tubulin stained CC was not significantly different between WT and nrca14 photoreceptors (p = 0.9 and 0.4 respectively). There was no significant difference (p = 0.7) in the number of IFT88 stained cilia between WT and nrca14 (J). Antibody staining is shown in yellow; nuclei were stained with Sytox Green (D, E) or Hoechst (G, H) and are shown in blue. Syn = photoreceptor synapses, IS = inner segment. Scale bar = 1 μm in A and B and 2 μm in C–F. Graphs show mean values, error bars are STDEV for multiple larvae.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ift88 Ab1-ift88 WT standard conditions Day 5 retinal cone cell photoreceptor connecting cilium IHC
Ab1-ift88 synj1a14/a14 standard conditions Day 5 retinal cone cell photoreceptor connecting cilium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT standard conditions Day 5 retinal cone cell photoreceptor connecting cilium
IHC synj1a14/a14 standard conditions Day 5 retinal cone cell photoreceptor connecting cilium
Phenotype Details
Fish Conditions Stage Phenotype
synj1a14/a14 standard conditions Day 5 retinal cone cell has normal numbers of parts of type retinal cone cell photoreceptor connecting cilium, normal
Day 5 retinal cone cell photoreceptor connecting cilium morphology, normal
Day 5 retinal cone cell photoreceptor outer segment length, normal
Day 5 retinal cone cell photoreceptor outer segment morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One