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ZFIN ID: ZDB-FIG-140324-20
Seritrakul et al., 2014 - Expression of the de novo DNA methyltransferases (dnmt3-dnmt8) during zebrafish lens development. Developmental dynamics : an official publication of the American Association of Anatomists   243(2):350-6 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4

Fig. 3

dnmt4 and dnmt7 expression in the lens epithelium and ciliary marginal zone. A–E,K: In situ hybridization shows broad dnmt4 expression in the developing eye at 24 hr postfertilization (hpf) (A), which later becomes localized in the CMZ and lens epithelium (B–E, K, arrow points to LE). dnmt4 expression is also observed in the tectum, developing pectoral fin, and pharyngeal arches (A–E). F: dnmt7 is expressed ubiquitously at 24 hpf. G–J,L: At 36 hpf, expression remains diffuse in the anterior part of the embryo, and surrounding of the eye, while expression within the eye is only observed in the CMZ and LE (L, arrow points to LE). Transverse sections, dorsal up (K,L).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dnmt3ba WT standard conditions Prim-5 eye ISH
Prim-5 head ISH
Prim-5 whole organism anterior region ISH
Prim-25 lens epithelium ISH
Prim-25 retina ISH
Prim-25 whole organism anterior region ISH
Long-pec ciliary marginal zone ISH
Long-pec lens epithelium ISH
Long-pec optic tectum ISH
Long-pec pectoral fin ISH
Long-pec pharyngeal arch ISH
Protruding-mouth ciliary marginal zone ISH
Protruding-mouth lens epithelium ISH
Protruding-mouth optic tectum ISH
Protruding-mouth pectoral fin ISH
Protruding-mouth pharyngeal arch ISH
Day 4 ciliary marginal zone ISH
Day 4 lens epithelium ISH
Day 4 optic tectum ISH
Day 4 pectoral fin ISH
Day 4 pharyngeal arch ISH
dnmt3bb.1 WT standard conditions Prim-5 eye ISH
Prim-5 head ISH
Prim-5 whole organism anterior region ISH
Prim-25 lens epithelium ISH
Prim-25 retina ISH
Prim-25 whole organism anterior region ISH
Long-pec ciliary marginal zone ISH
Long-pec lens epithelium ISH
Long-pec optic tectum ISH
Long-pec pectoral fin ISH
Long-pec pharyngeal arch ISH
Protruding-mouth ciliary marginal zone ISH
Protruding-mouth lens epithelium ISH
Protruding-mouth optic tectum ISH
Protruding-mouth pectoral fin ISH
Protruding-mouth pharyngeal arch ISH
Day 4 ciliary marginal zone ISH
Day 4 lens epithelium ISH
Day 4 optic tectum ISH
Day 4 pectoral fin ISH
Day 4 pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.