ZFIN ID: ZDB-FIG-140311-41
Fidler et al., 2014 - A unique covalent bond in basement membrane is a primordial innovation for tissue evolution. Proceedings of the National Academy of Sciences of the United States of America   111(1):331-336 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 4

Expression of collagen IV and Pxdn during development in zebrafish and morpholino (MO) knockdown of peroxidasin in zebrafish embryos. (A) Pxdn and collagen IV expression during zebrafish embryonic development. Real-time qPCR studies were conducted to examine expression levels of Pxdn, collagen4α1, and collagen4α2. *Student t test P value < 0.03 compared with expression at 1,000 cells. Error bars = SEM. Blue, pxdn; red, col4a; black, col4a2. (B) Control and (C) Pxdn MO groups. MO-injected embryos displayed (D) general severe defects that include cardiac edema, smaller eyes, and gross trunk patterning defects (4/45), (E) partial curved trunk (21/45), or (F) normal development (20/45). (G) SDS/PAGE analysis of Pxdn MO embryonic phenotypes at 24 hpf by Western blot. Collagenase digests were normalized for total protein load by protein stain with SYPRO-Ruby (Fig. S8).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col4a1 EKW standard conditions Prim-5 whole organism RTPCR
col4a2 EKW standard conditions Prim-5 whole organism RTPCR
pxdn EKW standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-col4 IHC EKW control Prim-5 whole organism collagen type IV trimer
IHC EKW + MO1-pxdn standard conditions Prim-5 whole organism collagen type IV trimer
Phenotype Details
Fish Conditions Stage Phenotype
EKW + MO1-pxdn standard conditions Prim-5 basement membrane assembly involved in embryonic body morphogenesis process quality, abnormal
Prim-5 eye decreased size, abnormal
Prim-5 heart edematous, abnormal
Prim-5 trunk curved dorsal, abnormal
Prim-5 whole organism collagen type IV trimer physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA