ZFIN ID: ZDB-FIG-140415-48
Fidler et al., 2014 - A unique covalent bond in basement membrane is a primordial innovation for tissue evolution. Proceedings of the National Academy of Sciences of the United States of America   111(1):331-336 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Prim-5

Fig. 3 NC1 hexamers were excised from animal basement membranes and analyzed by SDS/PAGE as shown in Fig. 1 A and B. The dimeric subunits, which indicate the presence of the bond, were found in nine major eumetazoan phyla. Among eight cnidarians investigated, only Hydra NC1 lacked dimeric subunits. All NC1s were immunoblotted against the rat monoclonal antibody, JK2, except for C. elegans (rabbit polyclonal; NW-154) and Drosophila (mouse monoclonal; 6G7). Black outlines indicate the locations of cropping for blot images.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-col4 IHC EKW standard conditions Prim-5 whole organism collagen type IV trimer
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA