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ZFIN ID: ZDB-FIG-140304-37
Zhang et al., 2014 - p35 promotes the differentiation of amacrine cell subtype in the zebrafish retina under the regulation of egr1. Developmental dynamics : an official publication of the American Association of Anatomists   243(2):315-23 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Fig. 4

The differentiation of GCs, BCs, rods, and MCs appeared normal in the p35-morphant retinas. A–D: Immunohistochemical analysis of GCs in the controls (stdCTLMO) and p35 morphants (p35tMO2) was performed with anti-zn8 (zn8) at 72 and 120 hpf. The zn8 signal is shown in green, while the plexiform layers are highlighted by phalloidin in red. At 72 hpf, the number of GCs, as counted by the DAPI nuclei with positive zn8, was not different between the control and p35-morphant retinas (A, B). This result suggests that the formation of GCs was not affected by p35 knockdown. Even though the dendritic projections of GC appeared compromised in the p35-morphant retinas at this stage, the overall zn8 staining pattern between the control and p35-morphant retinas was comparable at 120 hpf (C, D). These observations suggest that the GC differentiation was normal by this stage. E–J: Immunohistochemical analysis of BCs, rods, and MCs in the controls and p35 morphants was performed with anti-PKC βI (PKC), anti-zpr3 (zpr3), anti-GS (GS) at 72 hpf (data not shown) and 120 hpf. The p35 knockdown substantially reduced the staining signal of these markers in the p35-morphant retinas compared with the controls at 72 hpf. However, the staining became very comparable between the two conditions at 120 hpf. In these images, lateral is to left and dorsal is up. IPL: inner plexiform layer; OPL: outer plexiform layer. Scale bars = 50 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-8 IHC WT control Protruding-mouth retinal ganglion cell layer
IHC Day 5 retinal ganglion cell layer
IHC WT + MO2-cdk5r1b standard conditions Protruding-mouth retinal ganglion cell layer
IHC Day 5 retinal ganglion cell layer
zpr-3 IHC WT control Day 5 retinal rod cell
IHC WT + MO2-cdk5r1b standard conditions Day 5 retinal rod cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.