Expression pattern of snail1 in no tail embryos. (A,B) Posterior views of 5-somite stage; (C,D) lateral views of 12-somite stage; (A,C) wild-type embryos; (B,D) no tail embryos. Compared to a wild-type embryo (A), note the reduction of snail1 signal in no tail, especially in the tail bud, even though this no tail embryo was stained for 6 hours to be able to see a clear signal, and the wild-type control was stained for just 2 hours. The axial mesoderm territory is broader in no tail embryos. In contrast to wild type (C), an overstained no tail embryo shows an absence of snail1 staining in the tail. Since the mutant embryo was stained for a long time, background staining started to become visible.
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