pax-6 and eng-genes are expressed in adjacent regions. Adjacent horizontal sections of 14 h embryos were hybridized with probes specific for pax-6 or eng-1 separately or with pax-6 and eng-2 (A) or pax-6 and eng-1 (B, C) together. Anterior is to the left. Only sections with the double-hybridization are shown. Arrows delineate the limits of eng-1/2 expression. The signals to the left and to the right of eng-1 expression are specific for pax-6. The eng genes are expressed in the gap of pax-6 activity in the mes- and metencephalon. C is a higher magnification view of B. The eng-1 expression domain is separated from that of pax-6 by about 5 to 10 cells which show no activity of either gene. (D) Schematic summary of the superimposed expression domains. Stippled areas express pax-6, the dark areas express only eng-1 and the lightly stippled area expresses both eng-1 and eng-2. The broken line indicates the edge of the yolk. Scale bar is 200 μm in A, B, D and 80 μm in C.
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