rtk2 and msxC expression in wild-type, cyclops and shh-injected embryos. Whole-mounted embryos with rostral to the left (A,B,F-H) or up (D,E). (A,B) Lateral views of rtk2 expression in 24 hour wild-type (A) and cyclops mutant (B) embryos. The arrowheads indicate the boundary between nasal and temporal retina at the posterior groove. The asterisk and filled circle indicate equivalent positions of the eye in B and C. (C) Frontal view of rtk2 expression in a 20s cyclops mutant embryo. rtk2 expression in temporal retina is continuous around the rostral pole of the brain. (D,E) Dorsal views of rtk2 expression in 24 hour wild-type and shh-injected embryos respectively. rtk2 expression is widespread thoughout the retinal tissue in the shh-injected embryo. (F) Lateral views of msxC expression in the eye (arrowheads) of wild-type (right) and cyclops mutant (left) 18s embryos. (G,H) Lateral views of msxC expression in the eyes of shh injected (right) and wild-type (left) 18-20s (G) and 24 hour (H) embryos. In addition to an absence of msxC expression in the eyes (arrowheads), msxC is reduced throughout the head (arrows). Abbreviations: cf, choroid fissure; d, diencephalon; l, lens; nr, nasal retina; os, optic stalk; t, telencephalon; te, tectum; tr, temporal retina. Scale bars, 50 μm.
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