ZFIN ID: ZDB-FIG-140227-39
Macdonald et al., 1995 - Midline signalling is required for Pax gene regulation and patterning of the eyes. Development (Cambridge, England)   121:3267-3278
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Fig. 2 Pax protein distribution in wild-type and cyclops mutant embryos. Whole-mounted embryos labelled with anti-Pax6 antibody (A-F), or labelled with anti-Pax2 antibody (G,H). (A,B) Dorsal views of 15-16s wild-type and cyclops embryos respectively. The arrowheads in B indicate the bridge of rostral retinal fusion. (C,D) Dorsal and lateral (focused on the midline) views respectively of 20s cyclops embryos. The arrowheads indicate the area of retinal fusion in C. In D the lateral portions of the cyclopic eye have been removed. The arrowhead indicates the caudal limit of eye fusion. (E) Frontal view of 28s cyclops mutant embryo. (F) Lateral view of 28 hour cyclops mutant embryo also labelled with anti-tubulin antibody (black labelling of axons). This embryo was also examined in a different study (Macdonald et al., 1994). (G,H) 18- 20s dorsal views of wild-type (G) and cyclops (H) embryos labelled with anti-Pax2 antibody. The arrow in H indicates a few cells near the midline weakly labelled with anti-Pax2 antibody. Abbreviations: d, diencephalon; hb, hindbrain; mb, midbrain; mdf, mid-diencephalic furrow; l, lens; mt, midbrain tegmentum; ne, neural ectoderm; nr, nasal retina; os, optic stalks; np, nasal placode; op, optic primordia; ov, optic veiscle; ple, presumptive lens ectoderm; pnr, presumptive neural retina; ppe, presumptive pigment epithelium; se, surface ectoderm; t, telencephalon; te, tectum; tr, temporal retina; vd, ventral diencephalon. Scale bars, 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
pax2a Ab1-pax2a WT standard conditions 14-19 somites to 20-25 somites midbrain hindbrain boundary IHC
Ab1-pax2a 14-19 somites to 20-25 somites optic cup antero-ventral region IHC
Ab1-pax2a 14-19 somites to 20-25 somites optic stalk IHC
Ab1-pax2a ndr2Df(Chr12:hhex)b16/b16 standard conditions 14-19 somites to 20-25 somites midbrain hindbrain boundary IHC
Ab1-pax2a 14-19 somites to 20-25 somites Not Detected optic cup IHC
pax6a Ab2-pax6a WT standard conditions 14-19 somites diencephalon IHC
Ab2-pax6a 14-19 somites hindbrain IHC
Ab2-pax6a 14-19 somites optic vesicle IHC
Ab2-pax6a ndr2Df(Chr12:hhex)b16/b16 standard conditions 14-19 somites diencephalon IHC
Ab2-pax6a 14-19 somites hindbrain IHC
Ab2-pax6a 14-19 somites optic vesicle IHC
Ab2-pax6a 20-25 somites diencephalon IHC
Ab2-pax6a 20-25 somites hindbrain IHC
Ab2-pax6a 20-25 somites optic cup IHC
Ab2-pax6a Prim-5 diencephalon IHC
Ab2-pax6a Prim-5 presumptive neural retina IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.