Differential expression of the msxB gene along the proximodistal axis of the regenerating fin. (A) Amputation of a single zebrafish caudal fin at three different levels along the proximodistal axis. The skeletal elements of the fin are shown as in Fig. 5A. (B) msxB expression in blastema cells of regenerating rays of a fin which was partly amputated at three different levels. (C-E) Higher magnification photographs of the fin shown in B; (C) proximal cut; (D) intermediate cut; (E) distal cut. Gene expression was determined 4.5 d after amputation. The plane of amputation is indicated by a dashed line in A and by arrows in B,D,E. Scale bar: 500 μm in B; 100 μm in C-E.
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