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ZFIN ID: ZDB-FIG-140214-22
Begemann et al., 2000 - Developmental regulation of Tbx5 in zebrafish embryogenesis. Mechanisms of Development   90(2):299-304 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Day 5

Fig. 3

tbx5.1 expression in pectoral fin buds in wild type and mutant zebrafish. (A-E) tbx5.1 expression during wild type pectoral fin development. (A) 18-somite stage. Somites 1-4 are indicated. (B) 32 hpf. Arrow points to tbx5.1 expressing cells anterior to and outside of the pectoral fin bud. (C) 48 hpf. Expression is restricted to the distal part of the fin bud, which forms the fin endoskeleton proper. (D) 3 day embryo. The band of darker staining in the proximal mesenchyme is due to melanocytes that cover the epidermis at the ventral base of the fin bud. (E) 5 day embryo. Note the absence of tbx5.1 expression in the ectoderm of the apical fold and in the scapulocoracoid (sc). (F-I) tbx5.1 is expressed in dak mutant fin buds. (F) 36 hpf wild type and (G) daktw25 pectoral fin buds. (H, I) At 5 days, daktw25 mutants (I) lack differentiated pectoral fins. (J-Q) Maintenance of tbx5.1 in wild type (J-M) and syut4 mutant fin buds (N-Q). Expression at the 22-somite stage (J, N), 32 hpf (K, O), 36 hpf (L, M, P, Q). Anterior is to the left, (C-G, M, Q) lateral views with dorsal on top, (A, H-L, N-P) dorsal views. Scale bars, 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
tbx5a WT standard conditions 14-19 somites pectoral fin field ISH
20-25 somites pectoral fin field ISH
Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
Long-pec Not Detected fin fold pectoral fin bud ISH
Long-pec pectoral fin bud distal side ISH
Protruding-mouth mesenchyme pectoral fin ISH
Day 5 pectoral fin ISH
Day 5 Not Detected pectoral fin fold ISH
Day 5 Not Detected scapulocoracoid ISH
ext2tw25e/tw25e standard conditions Prim-25 pectoral fin bud ISH
shhaDf(Chr07)t4/t4 standard conditions 20-25 somites pectoral fin field ISH
Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ext2tw25e/tw25e standard conditions Prim-25 pectoral fin development arrested, abnormal
Day 5 pectoral fin absent, abnormal
shhaDf(Chr07)t4/t4 standard conditions Prim-25 pectoral fin development arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 90(2), Begemann, G. and Ingham, P.W., Developmental regulation of Tbx5 in zebrafish embryogenesis, 299-304, Copyright (2000) with permission from Elsevier. Full text @ Mech. Dev.