Fig. 1

zfGrx2 is essential for development of the vasculature. (A) Confocal microscopy of fli:EGFP transgenic embryos with or without zfGrx2 at 35 and 48 h postfertilization (hpf). DA, dorsal aorta; DLAV, dorsal longitudinal anastomotic vessel; ISV, intersegmental vessel; PC, parachordal sprouts. (Scale bars, 100 μm.) (B) Statistical analysis of A shows mean ± SEM, significance (***P < 0.01) was calculated by two-tailed Student?s t test, 50 pg WT or mutated zfGrx2 mRNA were injected per single egg for rescue experiments. (C) Statistical analysis of intersegmental vessel formation in fli:EGFP embryos, zfGrx2 knockdown embryos, and zfGrx2 knockdown embryos rescued with 15 ng/embryo zfSIRT1 mRNA (48 hpf) (Fig. S2), mean ± SEM, significance (*P < 0.05) was calculated by two-tailed Student?s t test. (D) Quantitative real-time PCR of zfSirt1 transcripts in WT and zfGrx2 knockdown embryos 48 hpf. Presented are mean ± SEM of triplicates of two independent experiments.