ZFIN Image: Bräutigam et al., 2013, Fig. 1

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Fig. 1

zfGrx2 is essential for development of the vasculature. (A) Confocal microscopy of fli:EGFP transgenic embryos with or without zfGrx2 at 35 and 48 h postfertilization (hpf). DA, dorsal aorta; DLAV, dorsal longitudinal anastomotic vessel; ISV, intersegmental vessel; PC, parachordal sprouts. (Scale bars, 100 μm.) (B) Statistical analysis of A shows mean ± SEM, significance (***P < 0.01) was calculated by two-tailed Student’s t test, 50 pg WT or mutated zfGrx2 mRNA were injected per single egg for rescue experiments. (C) Statistical analysis of intersegmental vessel formation in fli:EGFP embryos, zfGrx2 knockdown embryos, and zfGrx2 knockdown embryos rescued with 15 ng/embryo zfSIRT1 mRNA (48 hpf) (Fig. S2), mean ± SEM, significance (*P < 0.05) was calculated by two-tailed Student’s t test. (D) Quantitative real-time PCR of zfSirt1 transcripts in WT and zfGrx2 knockdown embryos 48 hpf. Presented are mean ± SEM of triplicates of two independent experiments.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA