FIGURE

Fig. 3

ID

ZDB-FIG-140130-30

Publication

Kochhan et al., 2013 - Blood Flow Changes Coincide with Cellular Rearrangements during Blood Vessel Pruning in Zebrafish Embryos

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Fig. 3

Endothelial cell apoptosis during CrDI pruning.

All images in lateral view with anterior to the left. (A) Stills of time-lapse imaging of Tg(kdrl:H2B-EGFP)^mu122, Tg(kdrl:Hsa.HRAS-mCherry)^s916 from 37-45 hpf. (A′) Tg(kdrl:H2B-EGFP)^mu122 channel only. White brackets indicate dorsal CrDI. Yellow arrow in (A) and (A′) at 42.25 hpf time point points to dying cell. (B) Stills of time- lapse imaging of Tg(kdrl:H2B-EGFP)^mu122; Tg(kdrl:Hsa.HRAS-mCherry)^s916 embryos from 36-48 hpf injected with bcl2 RNA. No dying cells are detectable in the CrDI. (B′) Tg(kdrl:H2B-EGFP)^mu122 channel only. (C) Brightfield images of bcl2 mRNA or uninjected embryos. (D) Acridine orange staining on bcl2 mRNA injected or control embryos. (E) Quantification of CrDI pruning at 48 hpf.

Expression Data

Genes:	EGFP mCherry
Fish:	mu122Tg; s916Tg
Anatomical Terms:	cranial division of the internal carotid artery cranial division of the internal carotid artery plasma membrane nasal ciliary artery nasal ciliary artery plasma membrane
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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