Fig. 1

Pruning of the Cranial Division of the Internal Carotid Artery (CrDI) during eye blood vessel development in zebrafish embryos.

Relative zebavidin mRNA quantification was performed with qRT-PCR (A) in adult zebrafish tissues and (B) in developing zebrafish (0-7 days post fertilization (dpf)). Expression analysis of each tissue in (A) are shown as the mean (error bar = SD) of the biological replicates. The two-sample t-test was used to compare the expression between female gonads and female gills (P = 0.002), male gonads and male gills (nonsignificant, NS), female gonads and male gonads (P = 0.023) and female gills and male gills (NS). Expression analysis of the developmental samples in (B) are shown as the mean (SD) of the biological replicates on 0 dpf and as the mean (SD) of the technical replicates on 1-7 dpf. All of the developmental samples were pooled from 15 to 50 individuals depending on the age of embryos. The two-sample t-test was used to compare the expression of 0 dpf and 1 dpf samples to later time points. A statistically significant difference in expression was found between the 0 dpf sample and all of the other samples (P < 0.0001 in all comparisons) and also between the 1 dpf sample and the samples of 2, 4, 5, 6 and 7 dpf (P < 0.05 in all comparisons). In both (A) and (B) the expression of zebavidin is normalized to EF1a expression. The insets in the upper right corners represent enlargements of parts of the actual figures. (C) Embryos, injected with a Morpholino blocking the translation initiation site or with a random control Morpholino, 24 h after injection in comparison with WT embryos. (D) Embryos 48 h after injection.