Fig. 5
- ID
- ZDB-FIG-140122-10
- Publication
- Iwasaki et al., 2013 - Expression of Arginine Vasotocin Receptors in the Developing Zebrafish CNS
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The epiphyseal and nucPC neurons but not the nucMLF nor T reticular neurons express avtr1a1 at approximately 48 hpf. (A) Lateral view (anterior left, dorsal up) of a z-stack of confocal images of an embryo double labeled for avtr1a1 and anti-acetylated α tubulin (axons) showing that the cluster II avtr1a1+ cells at the lateral base of the epiphysis (red, asterisk) project axons into the DVDT (green, arrowhead) and the avtr1a1+ cluster III cells (red, star) are adjacent to the PC (arrow). The avtr1a1+ cells seen ventrally are the neurons in the ventral forebrain and near the forebrain/tegmentum boundary (I and IV). Scale: 50 µm. (B) A single focal plane seen in a lateral view showing that the cluster III avtr1a1+ cells (red) appear to extend axons in the PC (green, arrows). Scale: 50 µm. (C) Ventral perspective of a pitx2c:egfp embryo labeled with a avtr1a1 riboprobe showing that the nucMLF neurons (green, bracket) do not express avtr1a1 (red). Arrowhead denotes the MLF; asterisk denotes the forebrain avtr1a1+ neurons. Scale: 100 µm. (D) Dorsal perspective (anterior left) of the hindbrain of an embryo double labeled for avtr1a1 riboprobe (red, bracket) and MAb RMO44 (green) showing that the posterior hindbrain avtr1a1+ cells are not the T reticular neurons. The arrowhead indicates avtr1a1+ cells in the otocyst. Scale: 100 µm. |
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Reprinted from Gene expression patterns : GEP, 13(8), Iwasaki, K., Taguchi, M., Bonkowsky, J.L., and Kuwada, J.Y., Expression of Arginine Vasotocin Receptors in the Developing Zebrafish CNS, 335-42, Copyright (2013) with permission from Elsevier. Full text @ Gene Expr. Patterns