FIGURE

Fig. 6

ID

ZDB-FIG-140117-42

Publication

Yogev et al., 2013 - eIF4EBP3L acts as a gatekeeper of TORC1 in activity-dependent muscle growth by specifically regulating Mef2ca translational initiation

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Fig. 6

Active eIF4EBP3L regulates myofibrilogenesis following inactivity.

Embryos were injected with the indicated MO, some exposed to MS222 for the last 17(A) or with plasmid DNA encoding a constitutively active eIF4EBP3L and GFP (5A3L-GFP) or empty vector (GFP) (B) were grown to 48–54 hpf and immunostained for slow MyHC. (A) BP3L MO1 prevents, and BP3L MO2 reduces, the decrease in slow MyHC caused by inactivity. Immunostaining (left panels) was quantified by confocal microscopy (right panel; n = 15 embryos/sample). (B) Overexpression of 5A3L reduces myofibril bundle width. Following heat shock, the size of slow myofibril bundles in GFP+ fibers was determined relative to their GFP neighbors (n = 9 embryos/sample). Bars represent SEM and samples were compared by t test. All experiments were repeated at least twice. Scale bar = 23 μm.

Expression Data

Antibody:	Ab-F59
Fish:	WT WT + MO2-eif4ebp3l
Condition:	chemical treatment: tricaine methanesulfonate
Knockdown Reagent:	MO2-eif4ebp3l
Anatomical Term:	slow muscle cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO2-eif4ebp3l
Condition:	chemical treatment: tricaine methanesulfonate
Knockdown Reagent:	MO2-eif4ebp3l
Observed In:	skeletal myofibril assembly slow muscle cell myosin complex
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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