ZFIN Image: Yogev et al., 2013, Fig. 6

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Figure Caption

Fig. 6

Active eIF4EBP3L regulates myofibrilogenesis following inactivity.

Embryos were injected with the indicated MO, some exposed to MS222 for the last 17(A) or with plasmid DNA encoding a constitutively active eIF4EBP3L and GFP (5A3L-GFP) or empty vector (GFP) (B) were grown to 48–54 hpf and immunostained for slow MyHC. (A) BP3L MO1 prevents, and BP3L MO2 reduces, the decrease in slow MyHC caused by inactivity. Immunostaining (left panels) was quantified by confocal microscopy (right panel; n = 15 embryos/sample). (B) Overexpression of 5A3L reduces myofibril bundle width. Following heat shock, the size of slow myofibril bundles in GFP+ fibers was determined relative to their GFP neighbors (n = 9 embryos/sample). Bars represent SEM and samples were compared by t test. All experiments were repeated at least twice. Scale bar = 23 μm.

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Acknowledgments

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