FIGURE

Fig. 5

ID

ZDB-FIG-140117-41

Publication

Yogev et al., 2013 - eIF4EBP3L acts as a gatekeeper of TORC1 in activity-dependent muscle growth by specifically regulating Mef2ca translational initiation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Overexpression of eIF4EBP3L reduces and knockdown rescues Mef2c.

Zebrafish embryos injected with RNA encoding eIF4EBP3L (A–C, F) or control MO or BP3L MO1 (D, E) were grown to 48–54 hpf and some exposed to MS222 for the last 17 h (A, B, E) or to 5 μM rapamycin for 6 h (Rap; A, C, F) or remained as untreated controls. (A–C) Mef2c immunostaining intensity quantified by confocal microscopy in 54 hpf embryos shows that overexpression of eIF4EBP3L reduced Mef2c protein in inactive (B) or rapamycin-treated (C) muscle. n = 60 (B) and n = 20 (C) embryos/sample. Scale bar = 90 μm. (D) Level of indicated mRNAs assayed by qPCR shows that BP3L morphants have reduced eif4ebp3l mRNA. (E) Western analysis shows that BP3L MO1 prevented decrease in Mef2c caused by inactivity compared to actin loading control. Bars represent SEM. Samples were compared by t test. All experiments were repeated at least twice. (F) Polysomal fractionation followed by triplicate qPCR for mef2ca and dmd mRNAs on polysomal and subpolysomal fractions.

Expression Data

Genes:	eif4ebp3l mef2ca
Antibody:	Ab2-mef2c
Fish:	WT WT + MO2-eif4ebp3l
Condition:	chemical treatment: tricaine methanesulfonate
Knockdown Reagent:	MO2-eif4ebp3l
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.