FIGURE

Fig. 4

ID

ZDB-FIG-140107-60

Publication

Weijts et al., 2013 - Atypical E2fs Control Lymphangiogenesis through Transcriptional Regulation of Ccbe1 and Flt4

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

E2f7/8 rescued Flt4-dependent lymphangiogenesis phenotypes.

A, Representative images of Tg(fli1a:gfp;flt1^enh:rfp) un-injected control embryos (nic) or embryos injected with e2f7/8 MOs or mRNA. B, C, D, E Quantification of the indicated parameters. Concentrations: e2f7/8 MOs (10 ng each); dll4 MO (3 ng); e2f7/8 mRNA (100 pg each); dll4 mRNA (100 pg).Open arrow heads indicate in (A; upper panel) hyper-branching of intersegmental vessels. Closed arrow heads indicate (upper panel in A) PLs or (lower panel, A) presence of the TD. All scale bars are 100 µm. Stars indicate missing TD fragments. Data presented as the average (±s.e.m.) compared to the control condition in three independent experiments (*** P<0.001). At least n = 150 embryos per condition in three independent experiments were used for A–E.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hu5333Tg; y1Tg + MO4-dll4 hu5333Tg; y1Tg + MO1-e2f7 + MO1-e2f8 + MO4-dll4
Knockdown Reagents:	MO1-e2f7 MO1-e2f8 MO4-dll4
Observed In:	intersegmental artery intersegmental vessel lymphangioblast lymphangioblast cord lymphangioblast lymphangiogenesis sprouting angiogenesis thoracic duct
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One