FIGURE

Fig. S3

ID

ZDB-FIG-140107-39

Publication

Fernandes et al., 2013 - Orthopedia Transcription Factor otpa and otpb Paralogous Genes Function during Dopaminergic and Neuroendocrine Cell Specification in Larval Zebrafish

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Fig. S3

High-resolution imaging of neuroendocrine cells for cell counting. Example of 3 dpf wildtype embryos imaged at single-cell resolution for quantification of cell numbers. (A) oxt, (B), avp, (C) trh, (D) crh expression analysis by WISH. For this figure, from the whole image stack with images at 1 μm spacing, sub-stacks of planes were used to generate a series of dorso-ventral Z-projections containing the region of interest. A higher magnification of regions of interest is shown on the right panel. Dorsal view, anterior at left. Scale bar is 100 μm. H, hypothalamus; HB, Hindbrain; PO, preoptic region; PT, posterior tuberculum.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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