IMAGE

Fig. S3

ID: ZDB-IMAGE-140107-35
Publication: Fernandes et al., 2013 - Orthopedia Transcription Factor otpa and otpb Paralogous Genes Function during Dopaminergic and Neuroendocrine Cell Specification in Larval Zebrafish
All Figures: Figures for Fernandes et al., 2013

Image

Figure Caption

Fig. S3 High-resolution imaging of neuroendocrine cells for cell counting. Example of 3 dpf wildtype embryos imaged at single-cell resolution for quantification of cell numbers. (A) oxt, (B), avp, (C) trh, (D) crh expression analysis by WISH. For this figure, from the whole image stack with images at 1 μm spacing, sub-stacks of planes were used to generate a series of dorso-ventral Z-projections containing the region of interest. A higher magnification of regions of interest is shown on the right panel. Dorsal view, anterior at left. Scale bar is 100 μm. H, hypothalamus; HB, Hindbrain; PO, preoptic region; PT, posterior tuberculum.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One