FIGURE

Fig. S4

ID

ZDB-FIG-131211-29

Publication

Phng et al., 2013 - Filopodia are dispensable for endothelial tip cell guidance

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

Latrunculin B disrupts F-actin polymerisation and endothelial cell morphology. Tg(Fli1ep:Lifeact-EGFP); Tg(Kdr-l:ras- Cherry)^s916 28.5 hpf embryos were treated with 2 μg/ml Lat. B. (A) At 20 minutes post-addition of Lat. B, endothelial tip cells of ISVs display multiple F-actin-filled filopodia (arrows) and junctional F-actin at the dorsal aorta (white arrows). (B-D) As drug treatment continued, fewer filopodia protrusions are observed. There is an increase in F-actin-positive puncta (black arrowheads) and loss of junctional F-actin (white arrowhead in D). Concomitant to perturbation of F-actin polymerisation, there is a change in EC morphology. The cell membrane becomes more convoluted at the cell body and at cell protrusions (magenta arrows). (E) Number of CAAX-positive filopodia after addition of 2 μg/ml Lat. B. n=2 ISVs from two embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development