FIGURE

Fig. S1

ID

ZDB-FIG-131210-19

Publication

Sheehan-Rooney et al., 2013 - Ahsa1 and Hsp90 activity confers more severe craniofacial phenotypes in a zebrafish model of hypoparathyroidism, sensorineural deafness and renal dysplasia (HDR)

Other Figures

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Fig. S1

Identification of a gata3 mutant zebrafish and expression of gata3. (A) The b1075 allele has a T>A point mutation (asterisk) in the 28^th bp of the fourth exon of gata3. The chromatograph shows a heterozygote. (B) The mutation generates a predicted C>S missense mutation in the conserved 2^nd Zinc Finger domain of Gata3 (G, Gallus gallus; H, Homo Sapien; M, Mus musculus; X, Xenopus laevis; D, Danio rerio, mut, b1075). (C) Schematic representation of the affected amino acid. (D-F) Embryos (E) injected with gata3 morpholino phenocopy (F) b1075 mutants. (G-J) Tissues disrupted in HDR express gata3. (G) Maxillary neural crest cells express gata3 (arrow). (H) Forming gill buds express gata3 by 72 hpf (arrows). (I) At 33 hpf, gata3 is expressed strongly in regions of the ear that will generate sensory cells (arrows). (J) Both the pronephric duct (pd) and Corpuscle of Stannius (cs) express gata3 at 33 hpf. Lateral views, anterior to the left. UIC=uninjected control.

Expression Data

Gene:	gata3
Fish:	WT
Anatomical Terms:	chondrocranium cartilage cranial neural crest corpuscles of Stannius otic vesicle macula pharyngeal pouch 3 pharyngeal pouch 4 pharyngeal pouch 5 pronephric duct
Stage Range:	Prim-15 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gata3^b1075/b1075 (EKW) WT + MO1-gata3
Knockdown Reagent:	MO1-gata3
Observed In:	embryonic neurocranium morphogenesis neurocranial trabecula neurocranium
Stage:	Day 5

Phenotype Detail

Acknowledgments

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