FIGURE

Fig. S29

ID

ZDB-FIG-131029-18

Publication

Garcia et al., 2013 - Engineering the Biosynthesis of the Polyketide-Nonribosomal Peptide Collismycin A for Generation of Analogs with Neuroprotective Activity

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Fig. S29

"Figure S29, related to Figure 5". Zebrafish assay results for compounds Collismycin DH, Collismycin C, Collismycin H2, Collismycin H3, Collismycin H4 and Collismycin H5.
(A): Induction of apoptosis in the zebrafish model visualized by fluorescence microscopy. The zebrafish larvae were treated with 10 μM retinoic acid alone as stress inducer (RA control) or in combination with 1 μM of the different collismycin analogs. As a positive neuroprotectant control zebrafish were treated with 10 μM RA and 1 μM lipoic acid (LA).
(B): Effect of the different collismycin analogs on retinoic acid-induced apoptosis in zebrafish larvae. Apoptosis induced by retinoic acid in the presence or absence of collismycin A and different collismycin analogs was determined by fluorescence microscopy and, after quantification, relative apoptosis was standardized using the level of apoptosis generated by the RA treatment as 100%. None of the analogs here represented exhibited significant protection.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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