FIGURE

Fig. 5

ID

ZDB-FIG-131029-17

Publication

Garcia et al., 2013 - Engineering the Biosynthesis of the Polyketide-Nonribosomal Peptide Collismycin A for Generation of Analogs with Neuroprotective Activity

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Fig. 5

Neuroprotective Action of the Collismycin Analogs
(A) Induction of apoptosis in a zebrafish model as visualized by fluorescence microscopy. The zebrafish larvae were treated with 10 μM RA alone as a stress inducer (RA control) or in combination with 1 μM of the different collismycin analogs. As a positive neuroprotectant control, zebrafish were treated with 10 μM RA and 1 μM LA. See also Table S2.
(B) Effect of the different collismycin analogs on RA-induced apoptosis in zebrafish larvae. Apoptosis induced by RA in the presence or absence of different collismycin analogs was determined with fluorescence microscopy and, after quantification, relative apoptosis was standardized using the level of apoptosis generated by the RA treatment as 100%. One-way ANOVA and Dunnett’s t test were used to determine the significance. A p value less than 0.05 was considered statistically significant. All analogs exhibited significant protection with p < 0.05. See also Table S1.
See also Figures S27–S29.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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