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Fig. 5

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ZDB-FIG-131017-22

Publication

Pashos et al., 2013 - Distinct enhancers of ptf1a mediate specification and expansion of ventral pancreas in zebrafish

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Fig. 5

ptf1a autoregulation is required for exocrine pancreas development. (A) The diagram indicates the endogenous ptf1a locus (top line). In the control BAC (middle line) the ptf1a coding sequence was modified by synonymous substitutions and the addition of a Kozak sequence (at bottom) resulting in a transcript resistant to the translation blocking MO. The Δ-5-3 BAC has an additional 2 kb deletion removing the autoregulatory enhancer. (B) At 5 dpf, the ptf1a:egfp BAC shows robust expression in exocrine pancreas, as well as some hindbrain expression. In fish without a rescuing BAC, MO injection abolished exocrine pancreas development at 5 dpf (C), while the presence of the control BAC expressing MO-resistant ptf1a allowed complete (D) or partial (F) pancreas development at the same stage. In contrast, the Δ-5-3 BAC failed to rescue ventral pancreas development (E), although hindbrain expression was apparently normal. Scale bars: 200 μm.

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 381(2), Pashos, E., Tae Park, J., Leach, S., and Fisher, S., Distinct enhancers of ptf1a mediate specification and expansion of ventral pancreas in zebrafish, 471-81, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.