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Fig. 2

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ZDB-FIG-131017-19

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Pashos et al., 2013 - Distinct enhancers of ptf1a mediate specification and expansion of ventral pancreas in zebrafish

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Fig. 2

Survey of ptf1a locus for regulatory activity. (A) The diagram indicates the non-coding sequences, ranging in size from 3 to13 kb and collectively spanning the region between 17 kb and +48 kb of the transcriptional start site of ptf1a, which were assayed for enhancer activity. The isolated sequences showed transcriptional activity at 1 dpf (B?F) and 3 dpf (G?K) in regions of the nervous system and retina overlapping endogenous ptf1a expression. The 17ptf1a element located upstream of the autoregulatory enhancer drives non-specific neuronal expression at 1 dpf (B) and 3 dpf (G). The +6ptf1a enhancer is active in the retina, cerebellum (black arrowhead), hindbrain and spinal cord at 1 dpf (C) and 3 dpf (H). The +11ptf1a enhancer is also active in the hindbrain, including the cerebellum (black arrowhead), the spinal cord and more weakly in the retina (D: 1 dpf, I: 3 dpf). The +24ptf1a element activates expression in certain rhombomeres of the hindbrain and in the spinal cord (black arrow) (E: 1 dpf, J: 3 dpf). The +36ptf1a element is active in the retina, hypothalamus (white arrowhead), fin buds and notochord (F: 1 dpf, K: 3 dpf). Scale bars: 200 μm.

Expression Data

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Phenotype Data

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Acknowledgments

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Reprinted from Developmental Biology, 381(2), Pashos, E., Tae Park, J., Leach, S., and Fisher, S., Distinct enhancers of ptf1a mediate specification and expansion of ventral pancreas in zebrafish, 471-81, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.