FIGURE

Fig. S4

ID

ZDB-FIG-131002-16

Publication

Kim et al., 2013 - Multi-organ Abnormalities and mTORC1 Activation in Zebrafish Model of Multiple Acyl-CoA Dehydrogenase Deficiency

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

Decreased oxygen flux in type III dxa^vu463 at 8 dpf. Oxygen flux was measured by comparing oxygen consumption over time in control siblings and homozygous dxa^vu463 mutants. After the measured O₂ concentration in the Oroboros O2k Oxygraph was stabilized, chambers were stirred at 100 rpm to allow recording of a new stable O₂ concentration. Stirrers were then turned off as constant stirring was deleterious to the zebrafish larvae. This process was repeated until no further decrement in O₂ concentration was measured and the fish were no longer motile. Average oxygen flux was then calculated from the change in O₂ concentration over time from the beginning of the experiment to the end. p<0.007, Each experiment was repeated 4 times using 10 larvae of each genotype for each measurement.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	etfa^vu463/vu463
Observed In:	whole organism mitochondrion
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.