ZFIN Image: Kim et al., 2013, Fig. S4

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Fig. S4

Decreased oxygen flux in type III dxa^vu463 at 8 dpf. Oxygen flux was measured by comparing oxygen consumption over time in control siblings and homozygous dxa^vu463 mutants. After the measured O₂ concentration in the Oroboros O2k Oxygraph was stabilized, chambers were stirred at 100 rpm to allow recording of a new stable O₂ concentration. Stirrers were then turned off as constant stirring was deleterious to the zebrafish larvae. This process was repeated until no further decrement in O₂ concentration was measured and the fish were no longer motile. Average oxygen flux was then calculated from the change in O₂ concentration over time from the beginning of the experiment to the end. p<0.007, Each experiment was repeated 4 times using 10 larvae of each genotype for each measurement.

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Acknowledgments

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