FIGURE

Fig. 4

ID

ZDB-FIG-130828-23

Publication

Mahony et al., 2013 - Pomalidomide is nonteratogenic in chicken and zebrafish embryos and nonneurotoxic in vitro

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Fig. 4

Neurotoxicity of Thalidomide, Lenalidomide, and Pomalidomide at potent, anti-inflammatory concentrations. Ventral temporal pieces of retinas from E14.5 mice embryos were cultured in the presence of DMSO/compound. (A–D) Following anti–β-tubulin staining and fluorescence imaging, the area of neurite outgrowth was analyzed following DMSO, Thalidomide 250 μg/mL (968 μM), Lenalidomide 200 μg/mL (772 μM), or Pomalidomide 60 μg/mL (219 μM) treatment. (E) Quantification of neurite outgrowth, indicating Pomalidomide has no effect on neurite outgrowth. Statistical significance was analyzed one-way ANOVA with Tukey’s post hoc test. Graphs represent mean ± SEM *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001; NS, P > 0.05. (Scale bar: 300 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA