FIGURE

Fig. S11

ID

ZDB-FIG-130827-26

Publication

Veldman et al., 2013 - Transdifferentiation of fast skeletal muscle into functional endothelium in vivo by transcription factor etv2

Other Figures

All Figure Page

Back to All Figure Page

Fig. S11

Etv2 cell autonomously initiates transdifferentiation of muscle cells. Blastula cell transplantation was performed from triple transgenic, mylpfa:mRFP/hsp70l:etv2/kdrl:GFP+, into wild-type embryos. Approximately 10 cells were transplanted per embryo. Transplanted embryos were raised until 22 hpf at which point they were selected for embryos displaying mylpfa:mRFP expression in distinct regions absent in kdrl:GFP. These embryos were then either heat shocked or left as no heat shock controls. Embryos were then analyzed for mylpfa:mRFP/kdrl:GFP coexpression at 10 h post–heat shock and followed out to 44 h post–heat shock. Two kdrl:GFP positive muscle fibers, one still mylpfa:mRFP positive (arrow), under-go transdifferentiation to form functional vessels supporting blood cell flow (see Movie S4).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.