FIGURE

Fig. 3

ID
ZDB-FIG-130808-27
Publication
Clancey et al., 2013 - Maintenance of Melanophore Morphology and Survival Is Cathepsin and vps11 Dependent in Zebrafish
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Fig. 3

Chromatophore specific gene expression occurs at expected times and in wildtype levels in vps11w66 mutants.

A, B) Dorsal head images of 2dpf wildtype and vps11w66 mutant larvae processed for melanophore specific dopachrome tautomerase (dct) expression. dct is detected in the correct place and time in vps11w66 mutants. C, D) Close up examination of dorsal head regions shown in A and B. Asterisks indicate reference cells for orientation. Melanin synthesis was inhibited in all in situ hybridization experiments using 1X 1-phenyl-2-thiourea. E, F) Dorsal images of wildtype and vps11w66 mutant trunk regions. In the trunk, dct is detected in expected levels but we observe uncharacteristic gaps between cells as compared to wildtype siblings. G) Quantification of dct+ cells (dorsal, lateral and ventral stripes) suggests an insignificant reduction in cell number at 2dpf, consistent with pigmented melanophore counts in 1J (p = 0.14 via two way ANOVA with Bonferonni multiple comparisons analysis). Error bars are standard deviation. H, I) Lateral tail images of 2dpf wildtype sibling or vps11w66 mutant larvae processed for iridophore specific purine nucleoside phosphorylase 4a (pnp4a) expression. Dorsal and ventral stripes are indicated in H by black and white arrowheads, respectively. J) Quantification of pnp4a+ cells in 2dpf wildtype and vps11w66 larvae suggest similar numbers of iridophore precursors at this stage (p = 0.85 via two way ANOVA with Bonferonni multiple comparisons).

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
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