FIGURE

Fig. 1

ID

ZDB-FIG-130807-19

Publication

Hochgreb-Hägele et al., 2013 - Laminin beta1a controls distinct steps during the establishment of digestive organ laterality

Other Figures

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Fig. 1

s804 mutants display defects in left-right asymmetry of endodermal organs. (A,A′) The liver (Li) and pancreas (Pa) in wild-type and s804 mutants are revealed by GFP expression in the enhancer trap line Tg(XlEef1a:GFP). Ventral views, anterior towards the top. (B,B2) myl7 and foxa3 expression in wild-type (B) and s804 mutants (B2), as revealed by whole-mount in situ hybridization. myl7 is expressed in cardiomyocytes and foxa3 is expressed in the gut primordium. Dorsal views. Dashed lines mark the midline. (C) Repartition of embryos according to cardiac asymmetry, as determined by myl7 expression. The numbers of embryos analyzed are indicated at the bottom. (D,D′) Confocal imaging projection of the liver (Li) and pancreas (Pa) at 72 hpf in wild-type (D) and s804 mutants (D′), as revealed by Tg(XlEef1a:GFP) expression (green). Ventral views. Islet 1 (red); pan-cadherin (blue). Dotted lines outline islet1-positive endocrine cells in the principal islet and throughout the hepatopancreatic ductal system. Scale bars: 20 μm.

Expression Data

Genes:	foxa3 GFP myl7
Antibodies:	Ab1-pan-Cadherin Ab2-isl
Fish:	WT lamb1a^s804/s804 s854Tg lamb1a^s804/s804; s854Tg
Anatomical Terms:	cardiac muscle cell intestinal bulb primordium liver pancreas pancreatic bud pancreatic system endocrine cell primary islet endocrine cell
Stage Range:	Prim-15 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lamb1a^s804/s804 lamb1a^s804/s804; s854Tg
Observed In:	determination of digestive tract left/right asymmetry determination of heart left/right asymmetry determination of liver left/right asymmetry determination of pancreatic left/right asymmetry intestinal bulb primordium liver liver primordium pancreas pancreas endocrine cell pancreas primordium
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

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