PUBLICATION

Laminin beta1a controls distinct steps during the establishment of digestive organ laterality

Authors
Hochgreb-Hägele, T., Yin, C., Koo, D.E., Bronner, M.E., and Stainier, D.Y.
ID
ZDB-PUB-130709-51
Date
2013
Source
Development (Cambridge, England)   140(13): 2734-2745 (Journal)
Registered Authors
Bronner-Fraser, Marianne, Stainier, Didier, Yin, Chunyue
Keywords
asymmetry, laminin, organogenesis
MeSH Terms
  • Functional Laterality/genetics
  • Functional Laterality/physiology
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Laminin/genetics
  • Laminin/metabolism*
  • Organogenesis/genetics
  • Organogenesis/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Animals
  • Immunohistochemistry
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Gastrointestinal Tract/embryology
  • Gastrointestinal Tract/metabolism
  • Cilia/metabolism
  • In Situ Hybridization
(all 21)
PubMed
23757411 Full text @ Development
Abstract

Visceral organs, including the liver and pancreas, adopt asymmetric positions to ensure proper function. Yet the molecular and cellular mechanisms controlling organ laterality are not well understood. We identified a mutation affecting zebrafish laminin β1a (lamb1a) that disrupts left-right asymmetry of the liver and pancreas. In these mutants, the liver spans the midline and the ventral pancreatic bud remains split into bilateral structures. We show that lamb1a regulates asymmetric left-right gene expression in the lateral plate mesoderm (LPM). In particular, lamb1a functions in Kupffer?s vesicle (KV), a ciliated organ analogous to the mouse node, to control the length and function of the KV cilia. Later during gut-looping stages, dynamic expression of Lamb1a is required for the bilayered organization and asymmetric migration of the LPM. Loss of Lamb1a function also results in aberrant protrusion of LPM cells into the gut. Collectively, our results provide cellular and molecular mechanisms by which extracellular matrix proteins regulate left-right organ morphogenesis.

Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m189
    Point Mutation
    pd24TgTransgenic Insertion
      s804
        Point Mutation
        s854TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          lamb1aMO2-lamb1aMRPHLNO
          notoMO3-notoMRPHLNO
          tbxtaMO1-tbxtaMRPHLNO
          1 - 3 of 3
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          Fish
          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          GFPEFGGFP
          1 - 2 of 2
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          Mapping
          No data available