FIGURE

Fig. 3

ID

ZDB-FIG-130719-14

Publication

Neal et al., 2013 - H. pylori Virulence Factor CagA Increases Intestinal Cell Proliferation by Wnt Pathway Activation in a Transgenic Zebrafish Model

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Fig. 3

CagA activates canonical Wnt signaling in the intestinal epithelium. (A?C) Quantitative RT-PCR data showing relative expression levels of the Wnt target genes mycA (A), cyclinD1 (B) and axin2 (C). Expression levels were assayed in dissected adult intestines and normalized to SDHA and β-actin; bars indicate mean ± s.d. of biological triplicates. (D?G) Immunofluorescence micrographs showing proliferating cells (EdU, green, 10-hour label) and cells with nuclear and cytoplasmic accumulation of β-catenin (red staining and white arrowheads) in intestinal cross-sections of wild-type (D), b-cagA (E), axin1^tm213 (F) and b-cagA; axin1^tm213 (G) animals at 6 dpf. (H) Quantification of proliferating (EdU+) cells. (I) Quantification of cells with nuclear and cytoplasmic accumulation of β-catenin.

Expression Data

Genes:	axin2 ccnd1 myca
Antibody:	Ab2-ctnnb1
Fish:	WT axin1^tm213/tm213 b1231Tg b1232Tg b1233Tg axin1^tm213/tm213; b1231Tg
Anatomical Terms:	intestinal epithelial cell cytoplasm intestinal epithelial cell nucleus intestine
Stage Range:	Day 6 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	axin1^tm213/tm213 axin1^tm213/tm213; b1231Tg
Observed In:	epithelial cell proliferation intestinal epithelial cell
Stage:	Day 6

Phenotype Detail

Acknowledgments

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