FIGURE

Fig. 5

ID

ZDB-FIG-130507-4

Publication

Grisham et al., 2013 - Mutations in ap1b1 Cause Mistargeting of the Na(+)/K(+)-ATPase Pump in Sensory Hair Cells

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Fig. 5

Decreased cell integrity and an increased number of intracellular membrane compartments in ap1b1 mutant HCs.

A, B, Comparable sections of the anterior macula of WT and tm246a mutant at 5 dpf. An asterisk indicates a breakdown of cytoplasm in a tm246a mutant HC. Scale bars, 3 μm. C, Example of an apical bleb extruding from a mutant HC containing several vesicular compartments and a multivesicular body. Scale bar, 1 μm. D–E, Comparable close-ups of WT and tm246a mutant HCs just below the cuticular plate. Scale bar, 1 μm. In tm246a mutant HCs, more multivesicular bodies (arrowheads) were present compared to WT HCs. F, Quantification of the observed number of multivesicular bodies in WT sibling and tm246a mutant HCs. G–H, Sections of HCs near the tight junctions showing an increased number of large vesicles in the mutants compared to WT. Vesicles in WT are indicated with arrows. I, Observed sizes of vesicles in WT sibling and tm246a mutant HCs. For quantification in F and I, WT: n = 15, tm246a: n = 20 HCs.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ap1b1^{tm246a/tm246a}
Observed In:	hair cell anterior macula hair cell anterior macula multivesicular body hair cell anterior macula vesicle
Stage:	Day 5

Phenotype Detail

Acknowledgments

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