ZFIN ID: ZDB-FIG-130429-16
Liu et al., 2013 - Direct and indirect roles of Fgf3 and Fgf10 in innervation and vascularisation of the vertebrate hypothalamic neurohypophysis. Development (Cambridge, England)   140(5):1111-1122 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth

Fig. 5 fgf3 expression and NH innervation and vascularisation in zebrafish. Lateral (A-K) or ventral (L-Q) views of wild-type zebrafish; anterior to the left. (A-C) fgf3 expression in posterior cells of the crabp1a+ NH (overlap in A indicated by arrow) and ventral-most cells of the posterior hypothalamus from 33 to 72 hpf. Dotted outline in B,C shows AH. (D,E) Double fluorescent in situ hybridisation reveals a partial overlap of fgf3 (green) and crapb1a (red) expression in posterior regions of the crabp1a domain at 32 hpf (D), whereas at 36 hpf, fgf3+ cells lie exclusively posterior of the crabp1a domain (E). (F,G) The crabp1a+ NH lies directly dorsal to the pomca+ AH. (H) Triple staining reveals innervation (AcTub) of NH (crabp1a) directly dorsal to AH (prl:RFP transgene product in lactotropes). Note that expression of AcTub and crabp1a signals are suboptimal owing to different fixation requirements. (I-N) Pioneering axons enter the anterior/dorsal NH anlage at 36 hpf (I,L). Axons are detected in the anterior half of the NH at 48 hpf (J,M), and throughout the NH at 72 hpf (K,N). Axons within the NH are indicated by arrows, axons outside the NH with arrowheads. For spatial reference, specimens are counterstained for the AH marker pomca. (O-Q) Endothelial cells (arrowhead) are detected at 36 hpf close to anterior regions of the pituitary (marked by pomca expression in AH) (O). At 48 hpf, endothelial cells assembling to vessels are detected anteriorly (arrowheads) and posteriorly (arrows) (P). At 72 hpf, the hypophyseal blood vessel system is completed (Q). ah, adenohypophysis; bca, basal communicating artery; cadi, caudal division of internal carotid artery; h, hours post fertilisation (hpf); hya, hypophyseal artery; hyc; hypophyseal capillary; hyv, hypophyseal vein; nh, neurohypophysis; oc, oral cavity; os, optic stalk. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
crabp1a WT standard conditions Prim-15 neurohypophysis ISH
Prim-25 neurohypophysis ISH
Long-pec neurohypophysis ISH
Protruding-mouth neurohypophysis ISH
zf113Tg standard conditions Protruding-mouth neurohypophysis ISH
fgf3 WT standard conditions Prim-15 hypothalamus ventral region ISH
Prim-15 neurohypophysis posterior region ISH
Prim-15 optic stalk ISH
Prim-25 hypothalamus ventral region ISH
Prim-25 Not Detected neurohypophysis posterior region ISH
Long-pec hypothalamus ventral region ISH
Long-pec Not Detected neurohypophysis posterior region ISH
Long-pec optic stalk ISH
Protruding-mouth hypothalamus ventral region ISH
Protruding-mouth Not Detected neurohypophysis posterior region ISH
Protruding-mouth optic stalk ISH
GFP s896Tg; zf44Tg standard conditions Prim-25 adenohypophyseal placode IHC
Long-pec adenohypophysis IHC
Protruding-mouth adenohypophysis IHC
zf44Tg standard conditions Prim-25 adenohypophyseal placode IHC
Long-pec adenohypophysis IHC
Protruding-mouth adenohypophysis IHC
mCherry s896Tg; zf44Tg standard conditions Prim-25 basal communicating artery blood vessel endothelial cell IHC
Prim-25 caudal division of the internal carotid artery blood vessel endothelial cell IHC
Long-pec basal communicating artery blood vessel endothelial cell IHC
Long-pec caudal division of the internal carotid artery blood vessel endothelial cell IHC
Long-pec hypophyseal artery blood vessel endothelial cell IHC
Protruding-mouth basal communicating artery blood vessel endothelial cell IHC
Protruding-mouth caudal division of the internal carotid artery blood vessel endothelial cell IHC
Protruding-mouth hypophyseal artery blood vessel endothelial cell IHC
Protruding-mouth hypophyseal capillary blood vessel endothelial cell IHC
Protruding-mouth hypophyseal vein blood vessel endothelial cell IHC
pomca WT standard conditions Prim-25 adenohypophyseal placode ISH
Long-pec adenohypophysis ISH
RFP zf113Tg standard conditions Protruding-mouth prolactin secreting cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC zf113Tg standard conditions Protruding-mouth neurohypophysis axon
IHC zf44Tg standard conditions Prim-25 neurohypophysis axon
IHC Long-pec neurohypophysis axon
IHC Protruding-mouth neurohypophysis axon
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development