Silencing of Stab1 expression in zebrafish inhibits assembly of parachordal lymphangioplasts (PLs).
(A) Overall morphology of 48 hpf zebrafish embryo after control morpholino injection. Red box shows region displayed in (B-D). (B) Normal formation of the PLs (arrows) in 48 hpf tg(fli1:EGFP) zebrafish embryo after injection of 4 ng control morpholino. (C,D) Silencing of Stab1 expression using 4 ng splice-blocking morpholino targeting exon 3 (C) or 12 ng splice-blocking morpholino targeting exon 4 (D) disrupted the formation of the PLs (asterisks) in 48 hpf tg(fli1:EGFP) fish embryos. (E) Quantification of 48 hpf tg(fli1:EGFP) fish embryos showing a disturbed PL formation. Embryos were divided in three groups depending on the PL appearance being completely absent, partially formed or completely present. (F) Functionality of the splice blocking morpholinos Stab1-Ex3-Mo and Stab1-Ex4-Mo. RT-PCR of control morpholino (4 ng), Stab1-Ex3-Mo (4 ng) and Stab1-Ex4-Mo (12 ng) injected zebrafish embryos at 24 hpf. WT: wild type, morph: morphant. Black scale bar: 500 μm. White scale bar: 50 μm.
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