header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-130423-39
Tay et al., 2013 - Lethal giant larvae 2 regulates development of the ciliated organ Kupffer's vesicle. Development (Cambridge, England)   140(7):1550-1559 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 10-13 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 10-13 somites to Long-pec

Fig. 3 Lgl2 is required for normal left-right asymmetry. (A) Asymmetric heart looping at 2 dpf visualized in Tg(cmlc2:GFP) transgenic embryos that express GFP in the heart. In control embryos, the heart (black arrow) looped to the right, whereas Lgl2 MO knockdown often resulted in reversed or no heart looping (red arrows). (B) Percentage of embryos with heart-looping defects. Injecting a lower dose of Lgl2 MO-1 revealed that looping defects are dose dependent, and co-injecting lgl2 mRNA partially rescued Lgl2 MO-1 and Lgl2 MO-2 heart defects. Injecting lgl2 mRNA alone also disrupted heart looping. (C) RNA in situ hybridization analysis of spaw expression, which was detected in left lateral plate mesoderm (LPM) (arrowhead) and bilaterally in the tail (arrows) at 14 hpf in controls. Lgl2 MO embryos showed disrupted LPM spaw expression, including right-sided, bilateral and absent expression (red arrowheads). (D) Analysis of LPM spaw expression in control and Lgl2 MO embryos. n, number of embryos analyzed. L, left; R, right.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg standard conditions Long-pec heart IHC
twu34Tg + MO1-llgl2 standard conditions Long-pec heart IHC
spaw AB/TU standard conditions 10-13 somites lateral plate mesoderm left side ISH
AB/TU + MO1-llgl2 standard conditions 10-13 somites lateral plate mesoderm left side ISH
10-13 somites lateral plate mesoderm right side ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TU + MO1-llgl2 standard conditions 10-13 somites determination of heart left/right asymmetry decreased process quality, abnormal
10-13 somites lateral plate mesoderm bilateral symmetry, abnormal
twu34Tg + MO1-llgl2 standard conditions Long-pec heart symmetry, abnormal
Long-pec heart looping decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development