FIGURE

Fig. 6

ID

ZDB-FIG-130412-6

Publication

Lu et al., 2013 - EpCAM Is an Endoderm-Specific Wnt Derepressor that Licenses Hepatic Development

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Fig. 6

EpCAM Derepresses Wnt Targets and Cooperatively Activates Wnt Signaling with Wnt Ligand(A) TOP-FLASH firefly luciferase reporter assays normalized by Renilla luciferase activities. TOP-FLASH, Tcf-β-catenin reporter; m, mouse. Error bars represent SD.(B–D) In contrast to the wild-type control (B, 33/35), expression of myca in the liver-forming area is reduced in hi2151 mutants (C, 23/30), which is rescued by epcam mRNA (D, 29/36).(E) EpCAM and Wnt3a at low concentrations cooperatively allow formation of LRP6-signalosomes. The quantities of plasmids used for transfections are 10% of those used in Figure 5C.(F) TOP-FLASH luciferase reporter assays. HEK293T is an EpCAM deficient cell line. Note that Wnt2bb and EpCAM can cooperatively activate the reporter. h, human. Error bars represent SD.See also Table S1.

Expression Data

Gene:	myca
Fish:	WT epcam^hi2151Tg; s854Tg
Anatomical Term:	liver primordium
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 24(5), Lu, H., Ma, J., Yang, Y., Shi, W., and Luo, L., EpCAM Is an Endoderm-Specific Wnt Derepressor that Licenses Hepatic Development, 543-553, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell