ZFIN Image: Lu et al., 2013, Fig. 6

Image

Figure Caption

Fig. 6 EpCAM Derepresses Wnt Targets and Cooperatively Activates Wnt Signaling with Wnt Ligand(A) TOP-FLASH firefly luciferase reporter assays normalized by Renilla luciferase activities. TOP-FLASH, Tcf-β-catenin reporter; m, mouse. Error bars represent SD.(B–D) In contrast to the wild-type control (B, 33/35), expression of myca in the liver-forming area is reduced in hi2151 mutants (C, 23/30), which is rescued by epcam mRNA (D, 29/36).(E) EpCAM and Wnt3a at low concentrations cooperatively allow formation of LRP6-signalosomes. The quantities of plasmids used for transfections are 10% of those used in Figure 5C.(F) TOP-FLASH luciferase reporter assays. HEK293T is an EpCAM deficient cell line. Note that Wnt2bb and EpCAM can cooperatively activate the reporter. h, human. Error bars represent SD.See also Table S1.

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Acknowledgments

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Reprinted from Developmental Cell, 24(5), Lu, H., Ma, J., Yang, Y., Shi, W., and Luo, L., EpCAM Is an Endoderm-Specific Wnt Derepressor that Licenses Hepatic Development, 543-553, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell