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ZFIN ID: ZDB-FIG-130411-31
Veerkamp et al., 2013 - Unilateral dampening of bmp activity by nodal generates cardiac left-right asymmetry. Developmental Cell   24(6):660-667 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 4 Control of Cardiac Progenitor Cell Motility by Bmp(A) Enrichment analysis of genes upregulated in Tg[hsp70l:bmp2b]fr13 versus WT cardiac tissue at 21.5 hpf according to Database for Annotation, Visualization and Integrated Discovery-based clustering for functional terms.(B) RT-qPCR comparative analysis of relative expression levels of myh9l2 in cardiac tissue-derived cDNAs at 24 hpf (mean values with SEM; p < 0.01; p < 0.0005).(C) Expression of phosphorylated NMII (pMLC2) is slightly higher on the right side of the WT cardiac field.(D) Strong expression of phosphorylated NMII throughout the entire heart cone in Tg[hsp70l:bmp2b]fr13 transgenic embryos heat shocked at 18 hpf.(E) Clonal misexpression of caMLCK on the left side of the cardiac cone affects cardiac laterality.(F and G) Loss of Ntl (F) and Spaw (G) abolishes cardiac laterality, but heart tube elongation is most strongly affected in spaw morphants. Heart tube length is the extension between the outflow tract region and leading edge of the atrium (arrows).(H) RT-qPCR comparative analysis of relative expression levels of myh9l2 in spaw and ntl morphant hearts at 24 hpf (mean values with SEM; p < 0.005).(I) Heart tube length in spaw and ntl morphants at 24 hpf (mean values with SEM; p < 0.0005).(J) Simulations of cardiac jogging based on a random cell motility model. Three representative simulations covering a range of L/R differences in cell motility and the respective bearing angles based on ten independent simulations for each condition are shown. The 9% condition of L/R motility differences most closely resembles WT cardiac jogging (red bearing angle).(K) Clonal misexpression of the Bmp antagonist Noggin1 within the left myocardium enhances the angle of cardiac jogging toward the left.(L) Model of Nodal-Bmp pathway interactions during stages of cardiac jogging.Total numbers of embryos indicate the occurrence of the most common phenotypes as shown. L, left; R, right; ns, not significant (for details on statistical analysis, see also Supplemental Experimental Procedures). Scale bars, 50 μm.See also Figures S3 and S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg + MO1-spaw standard conditions Prim-5 primitive heart tube IHC
twu34Tg + MO3-tbxta standard conditions Prim-5 primitive heart tube IHC
myh9a WT + MO1-spaw standard conditions Prim-5 primitive heart tube RTPCR
WT + MO3-tbxta standard conditions Prim-5 primitive heart tube RTPCR
fr13Tg heat shock Prim-5 primitive heart tube RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC twu34Tg + MO1-spaw standard conditions Prim-5 primitive heart tube
IHC twu34Tg + MO3-tbxta standard conditions Prim-5 primitive heart tube
Ab1-myl-Ser19-P IHC WT standard conditions 20-25 somites heart rudiment
IHC fr13Tg heat shock 20-25 somites heart rudiment
Phenotype Details
Fish Conditions Stage Phenotype
fr13Tg heat shock 20-25 somites BMP signaling pathway involved in heart development decreased process quality, abnormal
20-25 somites heart rudiment bilateral symmetry, abnormal
twu34Tg + MO1-spaw standard conditions Prim-5 determination of heart left/right asymmetry decreased process quality, abnormal
Prim-5 embryonic heart tube elongation decreased process quality, abnormal
Prim-5 primitive heart tube bilateral symmetry, abnormal
Prim-5 primitive heart tube decreased length, abnormal
twu34Tg + MO3-tbxta standard conditions Prim-5 determination of heart left/right asymmetry decreased process quality, abnormal
Prim-5 primitive heart tube bilateral symmetry, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 24(6), Veerkamp, J., Rudolph, F., Cseresnyes, Z., Priller, F., Otten, C., Renz, M., Schaefer, L., and Abdelilah-Seyfried, S., Unilateral dampening of bmp activity by nodal generates cardiac left-right asymmetry, 660-667, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell