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ZIRC
ZFIN ID: ZDB-FIG-130326-66
Zhang et al., 2013 - The Role of egr1 in Early Zebrafish Retinogenesis. PLoS One   8(2):e56108 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 5 Immunohistochemical analysis of the GCs in the Egr1-morphant retinas.

Immunohistochemical analysis of the GCs in the controls (5misCTLMO) and Egr1 morphants (egr1sMO) was performed by anti-zn8 (zn8; green) at 72 hpf (A & B) and 120 hpf (C & D). Phalloidin (red) was used as a counterstain to highlight the plexiform layers. A whole-eye section is shown at the top for each condition, while the magnified view of a selected region (white box) on the dorsal side of the optic nerve is shown at the bottom. The analysis has indicated that Egr1 knockdown suppressed the early dendritic outgrowth of GCs into the IPL at 72hpf (B), which was irregular at this stage. In addition, the cell number per retinal area was not different between the two groups. This defect was largely resolved by 120 hpf, despite the IPL was still thinner as shown in Figure 3. This suggests that there were still defects in differentiation of cells that projected neurites into the IPL. One possible cause of the defect is the differentiation problem of ACs as shown in Figure 4. See text, Table 1 and 2 for further discussion. For the whole-eye sections, the lens is on the left and dorsal is up. Scale bar = 50 µm for the whole-eye sections and 25 µm for the selected regions.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-8 IHC AB control Protruding-mouth retinal ganglion cell axon
IHC Protruding-mouth retinal ganglion cell dendrite
IHC Protruding-mouth retinal inner plexiform layer dendrite
IHC Day 5 retinal ganglion cell axon
IHC Day 5 retinal ganglion cell dendrite
IHC Day 5 retinal inner plexiform layer dendrite
IHC AB + MO6-egr1 standard conditions Protruding-mouth retinal ganglion cell axon
IHC Protruding-mouth retinal ganglion cell dendrite
IHC Protruding-mouth retinal inner plexiform layer dendrite
IHC Day 5 retinal ganglion cell axon
IHC Day 5 retinal ganglion cell dendrite
IHC Day 5 retinal inner plexiform layer dendrite
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO6-egr1 standard conditions Protruding-mouth retinal ganglion cell dendrite morphogenesis decreased occurrence, abnormal
Protruding-mouth retinal inner plexiform layer decreased thickness, abnormal
Protruding-mouth retinal inner plexiform layer has fewer parts of type retinal ganglion cell dendrite, abnormal
Day 5 retinal inner plexiform layer decreased thickness, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One