ZFIN ID: ZDB-FIG-130326-30
Ravi et al., 2013 - Sequencing of pax6 Loci from the elephant shark reveals a family of pax6 genes in vertebrate genomes, forged by ancient duplications and divergences. PLoS Genetics   9(1):e1003177 Full text @ PLoS Genet.
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Day 5
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 7 Characterization of zebrafish Pax6.2a.

A) rtPCR for Pax6.2a using cDNA from one to five dpf zebrafish embryos showing expression of the gene at all stages. rtPCR using Islet-1cDNA was used as control. B) Protein alignment of Pax6.2 from multiple species showing homology between the Pax6.2 proteins. Homology is highest in the homeobox but extends to several motifs in the PST domain. Zebrafish Pax6.2a shares highest homology with Pax6.2b proteins from other teleosts. (C–I) RNA in situ hybridisation analysis on fixed zebrafish embryos from 1 dpf to 5 dpf. C) At 24 hpf staining is seen in the head region of embryos. D) By 48 hpf expression has become limited to the developing retinae. E,F) Optical Projection Tomography (OPT) stills reveal the restricted expression of Pax6.2 in the retina where it appears limited to the inner nuclear layer. G–I) Expression in the inner nuclear layer of the retina is maintained at 3 dpf (G), 4 dpf (H) and 5 dpf (I). J) Morpholino depletion of Pax6.2a gives rise to zebrafish embryos with relatively smaller eyes compared to embryos injected with a control morpholino. Embryos were fixed at 2 dpf and tested for the presence of Pax6.2 transcript by in situ hybridization. K) Quantification of eye size reveals a 30% reduction in eye size relative to total body length in Pax6.2a morphants versus control morpholino injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
pax10 WT standard conditions Prim-5 head ISH
Prim-5 to Day 5 whole organism RTPCR
Long-pec retina ISH
Protruding-mouth retina ISH
Day 4 retina ISH
Day 5 retina ISH
WT + MO1-pax10 standard conditions Long-pec Not Detected retina ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-pax10 standard conditions Long-pec eye decreased size, abnormal
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.