ZFIN ID: ZDB-FIG-130318-10
Lu et al., 2013 - Failure in closure of the anterior neural tube causes left isomerization of the zebrafish epithalamus. Developmental Biology   374(2):333-344 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: 26+ somites to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: 26+ somites to Protruding-mouth

Fig. 1 flh is expressed in embryos with an open neural tube. In (A, E) WT and (B, F) sqt mutant embryos with closed neural tubes, the pineal precursors (p, white arrowheads) form a single, oval domain that spans the dorsal midline (dotted lines) separating the left (L) and right (R) sides of the epithalamus. (C) In sqt embryos with mild open neural tube defects, the pineal precursors still span the dorsal midline of the brain, but the pineal anlage is elongated along the left–right axis. (D, G, H) In sqt and cyc; sqt embryos with a severe open neural tube phenotypes, the pineal precursors are present in two divided domains on the left and right sides of the brain. Dorsal views of the epithalamus in (A–D) live or (E–H) fixed embryos, anterior to the top. Scale bar=20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP c162Tg standard conditions 26+ somites to Protruding-mouth pinealocyte IFL
ndr1cz35/cz35; c162Tg standard conditions 26+ somites to Protruding-mouth pinealocyte IFL
ndr1cz35/cz35; ndr2m294/m294; c162Tg standard conditions 26+ somites to Protruding-mouth pinealocyte IFL
noto WT standard conditions 26+ somites to Protruding-mouth pinealocyte ISH
ndr1cz35/cz35 standard conditions 26+ somites to Protruding-mouth pinealocyte ISH
ndr1cz35/cz35; ndr2m294/m294 standard conditions 26+ somites to Protruding-mouth pinealocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ndr1cz35/cz35 standard conditions 26+ somites - Protruding-mouth epiphysis morphology, abnormal
26+ somites - Protruding-mouth epiphysis split bilaterally, abnormal
26+ somites - Protruding-mouth neural tube open, abnormal
ndr1cz35/cz35; c162Tg standard conditions 26+ somites - Protruding-mouth epiphysis morphology, abnormal
26+ somites - Protruding-mouth epiphysis split bilaterally, abnormal
26+ somites - Protruding-mouth neural tube open, abnormal
ndr1cz35/cz35; ndr2m294/m294 standard conditions 26+ somites - Protruding-mouth epiphysis split bilaterally, abnormal
26+ somites - Protruding-mouth neural tube open, abnormal
ndr1cz35/cz35; ndr2m294/m294; c162Tg standard conditions 26+ somites - Protruding-mouth epiphysis split bilaterally, abnormal
26+ somites - Protruding-mouth neural tube open, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 374(2), Lu, P.N., Lund, C., Khuansuwan, S., Schumann, A., Harney-Tolo, M., Gamse, J.T., and Liang, J.O., Failure in closure of the anterior neural tube causes left isomerization of the zebrafish epithalamus, 333-344, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.